Below you find all protocols used in the GFN laboratory training courses. The protocols have been organized by subject for your convenience, and also complete sets of protocols for the different levels of GFN training courses can be found.
Additional protocols related to antimicrobial resistance, e.g. specific PCR-protocols (e.g. mcr-1, mecA, mecC), or protocols for isolation of ESBL-, AmpC-, and carbapenemase-producing E. coli from meat or caecal samples are available via the EURL-AR website (

Antimicrobial Susceptibility Testing
Susceptibility Testing of Enterobacteriaceae using Disc Diffusion
Antimicrobial Susceptibility Testing - E-test
Antimicrobial Susceptibility Testing - Microbroth Dilution
Antimicrobial Susceptibility Testing - Agar Dilution
Isolation of Salmonella spp. from Food and Animal Faeces
Isolation of Salmonella and Shigella from Faecal Specimens
PDF document, 500 KB
Salmonella/Shigella - Identification
Salmonella/Shigella - Identification (biochemical)
Biochemical identification of Salmonella/Shigella using an abbreviated panel of tests - version 002, updated October 2015 
(PDF document, 800 KB)
Salmonella - Identification (API)
Salmonella - Identification (API)
(PDF document, 605 KB)
Salmonella - Serotyping
Salmonella - Serotyping
(PDF document, 339 KB)
Shigella - SerotypingShigella - Serotyping
(PDF document, 715 KB)
Campylobacter - Isolation (faeces)
Campylobacter - Isolation (food)
Campylobacter - Isolation (food)
(PDF document, 329 KB)
Campylobacter - Isolation (water)
Campylobacter - Isolation (water)
(PDF document, 341 KB)
bacter - Identification
Campylobacter - Identification
(PDF document, 566 KB)
Campylobacter - Identification (PCR)
E. coli O157
   E. coli - Isolation and Identification
E. coli - Agglutination
E. coli - Agglutination
(PDF document, 390 KB)
E. coli - Agglutination (Latex)
E. coli - Agglutination (Latex)
(PDF document, 394 KB)
E. coli - Identification (PCR)
E. coli - Identification (PCR)
(PDF document, 732 KB)
A protocol on MLVA of Salmonella enterica serotype Typhimurium is available on the ECDC website:
Pathogenic E. coli
Methods for Detection and Molecular Characterisation of Pathogenic Escherichia coli
Protocols on Pulsed Field Gel Electroforesis are available on the PulseNet International website: