Search
WHO COLLABORATING CENTRE – ANTIMICROBIAL RESISTANCE
  
 
 
PROTOCOLS
 
Below you find all protocols used in the GFN laboratory training courses. The protocols have been organized by subject for your convenience, and also complete sets of protocols for the different levels of GFN training courses can be found.
  
 
 
Antimicrobial Susceptibility Testing
  
Susceptibility Testing of Enterobacteriaceae using Disc Diffusion
Antimicrobial Susceptibility Testing - E-test
Antimicrobial Susceptibility Testing - Microbroth Dilution
Antimicrobial Susceptibility Testing - Agar Dilution
 
 
 
Salmonella/Shigella
   
Isolation of Salmonella spp. from Food and Animal Faeces
Isolation of Salmonella and Shigella from Faecal Specimens
PDF document, 500 KB
Salmonella/Shigella - Identification
Salmonella/Shigella - Identification (biochemical)
Biochemical identification of Salmonella/Shigella using an abbreviated panel of tests (PDF document, 800 KB)
 
Salmonella - Identification (API)
Salmonella - Identification (API)
(PDF document, 605 KB)
Salmonella - Serotyping
Salmonella - Serotyping
(PDF document, 339 KB)
 
Shigella - SerotypingShigella - Serotyping
(PDF document, 715 KB)
 
 
 
Campylobacter
   
Campylobacter - Isolation (faeces)
Campylobacter - Isolation (food)
Campylobacter - Isolation (food)
(PDF document, 329 KB)
Campylobacter - Isolation (water)
Campylobacter - Isolation (water)
(PDF document, 341 KB)
bacter - Identification
Campylobacter - Identification
(PDF document, 566 KB)
Campylobacter - Identification (PCR)
 
 
 
E. coli O157
   E. coli - Isolation and Identification
E. coli - Agglutination
E. coli - Agglutination
(PDF document, 390 KB)
E. coli - Agglutination (Latex)
E. coli - Agglutination (Latex)
(PDF document, 394 KB)
E. coli - Identification (PCR)
E. coli - Identification (PCR)
(PDF document, 732 KB)
 
 
 
MLVA
 
A protocol on MLVA of Salmonella enterica serotype Typhimurium is available on the ECDC website: http://ecdc.europa.eu/en/publications/Publications/Forms/ECDC_DispForm.aspx?ID=748
 
 
Pathogenic E. coli
  
Methods for Detection and Molecular Characterisation of Pathogenic Escherichia coli
  
 
PFGE
  
Protocols on Pulsed Field Gel Electroforesis are available on the PulseNet International website: http://www.pulsenetinternational.org/protocols/Pages/default.aspx
 
 
Training Course - Level 1
 
 Isolation of Salmonella spp. from Food and Animal Faeces

Isolation of Salmonella and Shigella from Faecal Specimens
(PDF document, 500 KB)
Salmonella/Shigella - Identification
Salmonella/Shigella - Identification (biochemical)
Biochemical identification of Salmonella/Shigella using an abbreviated panel of tests (PDF document, 800 KB)
 
Salmonella - Identification (API)
Salmonella - Identification (API)
(PDF document, 605 KB)
Salmonella - Serotyping
Salmonella - Serotyping
(PDF document, 339 KB)
Susceptibility Testing of Enterobacteriaceae using Disc Diffusion
Antimicrobial Susceptibility Testing - E-test
Antimicrobial Susceptibility Testing - Microbroth Dilution
 
 
 
Training Course - Level 2
  
Salmonella - Serotyping
Salmonella - Serotyping
(PDF document, 339 KB)
Campylobacter - Isolation (faeces)
Campylobacter - Isolation (food)
Campylobacter - Isolation (food)
(PDF document, 329 KB)
Campylobacter - Isolation (water)
Campylobacter - Isolation (water)
(PDF document, 341 KB)
Campylobacter - Identification
Campylobacter - Identification
(PDF document, 566 KB)
Susceptibility Testing of Enterobacteriaceae using Disc Diffusion
Antimicrobial Susceptibility Testing - Agar Dilution
  
  
  
Training Course - Level 3+
  
Salmonella - Serotyping
Salmonella - Serotyping
(PDF document, 339 KB)
 
PFGE on Salmonella and E. coli (Protocols on PFGE are available on the PulseNet International website: http://www.pulsenetinternational.org/protocols/Pages/default.aspx
 
Campylobacter - Identification
Campylobacter - Identification
(PDF document, 566 KB)
Campylobacter - Identification (PCR)
 
 
 
Training Course - Level 4
  
Salmonella - Serotyping
Salmonella - Serotyping
(PDF document, 339 KB)
E. coli - Isolation and Identification
E. coli - Agglutination
E. coli - Agglutination
(PDF document, 390 KB)
E. coli - Agglutination (Latex)
E. coli - Agglutination (Latex)
(PDF document, 394 KB)
E. coli - Identification (PCR)
E. coli - Identification (PCR)
(PDF document, 732 KB)
 
 
 
Training Course - Level 5
  
PFGE on Salmonella and E. coli (Protocols on PFGE are available on the PulseNet International website: http://www.pulsenetinternational.org/protocols/Pages/default.aspx